Reprogramme-induced genomic stability

doi:10.3969/j.issn.2095-4344.2014.10.023

Abstract

Abstract:

BACKGROUND: Some studies have shown that more copy number variations are present in early passage human induced pluripotent stem cells than later passage human human induced pluripotent stem cells, their parental somatic fibroblasts or human embryonic stem cells.
OBJECTIVE: To investigate whether the reprogramming process itself compromises genomic stability and further explore the efficiency of induced pluripotent stem cell establishment.
METHODS: Using high-resolution Affymetrix CytoScan HD array, we compared copy number variations and loss of heterozygosity in early passage induced pluripotent stem cells with their fibroblast cell origins from genetic epilepsy patients.
RESULTS AND CONCLUSION: Compared with somatic fibroblasts from genetic epilepsy patient, there was no difference in the loss of heterozygosity between the two types of cells, but more copy number variations were present in early passage human induced pluripotent stem cells which were characterized as microduplication and involved oncogenic genes. Results demonstrate the dynamic nature of genomic abnormalities during reprogramming process and the necessity of frequent monitoring human induced pluripotent stem cells to assure their genomic stability and clinical safety.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: induced pluripotent stem cells, DNA copy number variations, genomics, loss of heterozygosity, epilepsy

CLC Number:

R394.2

Cao Ding-ya, Li Jie-liang, Liu Wei-qiang, He Wen-yin, He Wen-zhi, Luo Yu-mei, Fan Yong, Sun Xiao-fang. Reprogramme-induced genomic stability[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(10): 1621-1628.

Figures/Tables 11

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